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Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年05期
Page:
125-128
Research Field:
Publishing date:

Info

Title:
Identification of the Pathogen of Sclerotium rolfsii on Betonica officinalis and Screening of Antagonistic Trichoderma Strain
Author(s):
LIU Wei; LAN Zu-zai; YE Yun-feng; JIANG Ni; LIU Li-hui
Guangxi Botanical Garden of Medicinal Plant，Guangxi Medicinal Resources Conservation and Genetic Improvement Laboratory，Nanning，Guangxi 530023
Keywords:
Betonica officinalis; Sclerotium rolfsii; identification of the pathogen; antagonistic trichoderma strain; screen
PACS:
S 567.23+9
DOI:
-
Abstract:
Taking diseased plants of Betonica officinalis as experimental materials，the pathogen was identified by pathogenicity test；the types of pathogen were tested by the way of morphological identification and ITS rDNA sequence analysis；the antagonistic strains had been screened by confronting cultivation on PDA plates.The results showed that the pathogen of sclerotiniose on Betonica officinalis was identified as Sclerotium rolfsii Sacc；among the five tested trichoderma strains，Trichoderma harzianum H2 had the best control potential for controlling the sclerotiniose on Betonica officinalis，its antagonistic index and control efficiency were Ⅱ level and 86.27%，respectively.

References:

[1]冉先德.中华药海(下册)[M].哈尔滨：哈尔滨出版社,1993:1297.
[2]方中达.植病研究方法[M].北京:中国农业出版社,1998:194.
[3]魏景超.真菌鉴定手册[M].上海:上海科技出版社,1979:645-648.
[4]易润华,朱西儒,周而勋.简化CTAB法快速微量提取丝状真菌DNA[J].湛江海洋大学学报,2003,23(6):72-73. [5]曹玉桃,姚革,文成敬,等.木霉拮抗黄瓜枯萎病菌菌株的筛选[J].西南农业学报,2007,20(3):408-411.
[6]张丽荣,康萍芝,沈瑞清.土壤菌对土传病害病原真菌的拮抗作用[J].内蒙古农业科技,2007(5):48-50.
[7]金苹,高晓余.白绢病的研究[J].农业灾害研究,2011(1):14-22.
[8]戴芳澜.中国真菌总汇[M].北京:科学出版社,1979:1060.

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Last Update: 2014-08-22