|Table of Contents|

Study on Callus Induction and Subculture of Lonicera japonica Thunb.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年05期
Page:
106-109
Research Field:
Publishing date:

Info

Title:
Study on Callus Induction and Subculture of Lonicera japonica Thunb.
Author(s):
SHAN Guang-fu1; 2; ZHANG Xiao-li1; 3; LI Ming-jun1; 3; 4; CHEN Ming-xia1; 3; LIU Wen-ying1; LU Jie1
1.College of Life Science，Henan Normal University，Xinxiang，Henan 453007；
2.Henan Education Institute，Zhengzhou，Henan 450046；
3.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs，University of Henan Province，Xinxiang，Henan 453007;
4.Engineering Technology Research Center of Nursing and Utilization of Chinese Crude Drugs，Xinxiang，Henan 453007
Keywords:
Lonicera japonica Thunb.; callus; induction; subculture
PACS:
S 567.7+9
DOI:
-
Abstract:
Taking Lonicera japonica Thunb.‘Jinfeng No.1’ as material，the effect of different explants and different concentrations of plant growth regulators treatments on callus induction and subculture were studied.The results showed that the callus induction of the best explants was lower buds，and the best induction medium was MS＋NAA 2.0 mg/L＋6-BA 0.01 mg/L＋2,4-D 0.5 mg/L，the inducing ratio could reach 100%；the callus grew faster，plenty，moistly，loose，colors brightly.The best subculture medium for callus was MS＋6-BA 2 mg/L＋KT 0.75 mg/L＋NAA 0.25 mg/L，4 kinds of callus could be obtained after subcultured for 3~5 generations.

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Last Update: 2014-08-22