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Cloning of Betaine Aldehyde Dehydrogenase cDNA in Liqusticum chuanxiong and  Construction of its Plant Expression Vector(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年05期
Page:
87-90
Research Field:
Publishing date:

Info

Title:
Cloning of Betaine Aldehyde Dehydrogenase cDNA in Liqusticum chuanxiong and  Construction of its Plant Expression Vector
Author(s):
MAO Ying1; ZHANG Yan-jun1; WANG Wan-jun1; CHEN Jin-song2; LIAO Hai2; ZHOU Jia-yu2
1.College of Life Science and Engineering，Southwest Jiaotong University，Chengdu，Sichuan 610031；
2.Chengdu Institute of Biology，Chinese Academy of Sciences，Chengdu，Sichuan 610041
Keywords:
Liqusticum chuanxiong; betaine aldehyde dehydrogenase; cloning; plant expression vector
PACS:
S 567.23+9
DOI:
-
Abstract:
Taking Liqusticum chuanxiong as material，the total RNA was extracted from leaf of  Liqusticum chuanxiong and cDNA of Betaine aldehyde dehydrogenase gene were obtained using RT-PCR.The purified RT-PCR products were constructed into pMD18-T vector.The results showed that the full length of  BADH gene consists of 1 527 bp，which encoded 508 amino acids.The homology of its nucleotide sequence with HM35276 was 100%.Furthermore，the aim gene was cloned into plant expression vector pBI121 and a recombinant plasmid pBI121/Betaine aldehyde dehydrogenase were constructed successfully according to double enzyme digestion，PCR and sequencing.

References:

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Last Update: 2014-08-21