Research on the Extraction of Polysaccharides from Stipe Base of Lentinus edode and Function of Scavenging of DPPH Radical by the Polysaccharides(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Research on the Extraction of Polysaccharides from Stipe Base of Lentinus edode and Function of Scavenging of DPPH Radical by the Polysaccharides

Author(s):: ZHAO Xin; WU Zi-long; CHEN Li-feng; ZHAO Jiu-e; Department of Biology，Handan College，Handan Key Laboratory of Resource Plant，Handan，Hebei 056005

Keywords:: stipe base of Lentinus edode; polysaccharides; cellulose; 2; 2-diphenyl-1-picrylhydrazyl radical

Abstract:: Taking the stipe base of Lentinus edode and cellulose as materials，the optimum extraction process of polysaccharides from stipe base of Lentinus edode were studied using cellulose method.The results showed that the optimum conditions for extraction process were as follows，the powder size of L.edode 200 mesh，extracting temperature 35℃，extracting time 3 h，and the extraction rate of polysaccharides could reach 5.124%.The preliminarily purified polysaccharides had powerful scavenging efficiency to DPPH radical.Its IC₅₀ was 0.13 mg/mL.

[1] 田光辉 . 香菇多糖提取工艺的优化 [J]. 延安大学学报 ,2002(4):46-48.

[2]张润光.香菇的营养保健功能及其产品开发[J].食品研究与开发,2004(4):125-128.

[3]杜荣骞.生物统计学[M].北京:高等教育出版社,2009:307-314.

[4]周斌,林松毅,李超,等.超声波法提取香菇培养基废弃物中活性多糖的研究[J].食品科学,2008,29(11):237-240.

[5]张桂,赵国群.超声波萃取植物多糖的研究[J].食品科技,2005,26(9):302-304.

[6]李姣娟,周尽花,戴瑜,等.川桂叶总黄酮清除DPPH·自由基作用的研究[J].中南林业科技大学学报,2010,30(10):125-128,132.

[7]张志国,陈锦屏,邵秀芝,等.红枣核类黄酮清除DPPH自由基活性研究[J].食品科学,2007,28(2):67-70.

Last Update: 2014-09-01