|Table of Contents|

Establishment of Highly Efficient in vitro Plant Regeneration of Pyrethrum cinerariaefolium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年24期
Page:
135-139
Research Field:
Publishing date:

Info

Title:
Establishment of Highly Efficient in vitro Plant Regeneration of Pyrethrum cinerariaefolium
Author(s):
YU YaQU Ling
Department of Bioengineering,Wuhan Bioengineering Institute,Wuhan,Hubei 430415
Keywords:
Pyrethrum cinerariaefoliumadventitious budsbrowning
PACS:
S 682.1+1
DOI:
-
Abstract:
Using pyrethrum cinerariaefolium leaves as experimental materials,the factors of adventitious buds induction,shoot buds proliferation and shoots rooting were investigated systematically.The results showed that the effects of leaf sterilized with sodium hypochlorite were suitable than those with mercuric chloride.Leaf was sterilized in choice sodium hypochlorite for 8 minutes and the survival rate reached as high as 95%;The suitable medium of adventitious buds induction was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L,which made the shoot buds differentiation frequency of 83.3% and the average number of shoot buds per explants was 8.15.The shoots proliferation medium was MS+6-BA 1.0 mg/L+NAA 0.5mg/L,with the ratio of buds propagation was 9.16.The optimal rooting medium was MS without no hormones and the rooting rate of 80%.

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Last Update: 2014-09-01