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Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年24期
Page:
132-135
Research Field:
Publishing date:

Info

Title:
Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L.
Author(s):
Dilraba·DILXAT; Muhrapiya·ARKIN; ZHANG Fu-chun; ZHANG Xia
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering，College of Life Science and Technology,Xinjiang University，Urumqi，Xinjiang 830046
Keywords:
Brassica rapa L.; cotyledon; hypocotyl; callus; regeneration plants
PACS:
S 631.3
DOI:
-
Abstract:
The regeneration system of tissue culture of Xinjiang Brassica rapa L. was discussed by using cotyledon and hypocotyl of 5 day-old seedling of Xinjiang Brassica rapa.L as the explant.The results showed that MS+2,4-D 2.0 mg/L+KT 0.1 mg/L medium was effective for callus induced，the averaging callus frequences were 65.4%，95.5% for cotyledon，hypocotyl；shoots were induced with 6-BA 3.0 mg/L+NAA 0.5 mg/L+AgNO3 5.0 mg/L，the averaging regeneration shoots frequence was 20.8%，65.0% for cotyledon，hypocotyl；shoots was transferred into medium of MS+IBA 0.5 mg/L to root；after 20 days，regeneration plants were transferred into flower-soil and its survivable rate was more than 95%.

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Last Update: 2014-09-01