Study on Detection of Pepper Mild Mottle Virus by RT-PCR(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年24期

Page:

127-129

Research Field:

Publishing date:

Info

Title:

Study on Detection of Pepper Mild Mottle Virus by RT-PCR

Author(s):

ZHENG Min¹; GUO Shi-hui¹; ZHANG Na-na¹; YANG Hong-yi¹; 2

1.College of Life Science，Northeast Forestry University，Harbin，Heilongjiang 150040；

2.Northeast Agricultural University，Harbin，Heilongjiang 150030

Keywords:

pepper mild mottle virus; inoculate mechanically; RT-PCR

PACS:

S 436

DOI:

-

Abstract:

A number of peppers infected virus were found by field survey.The virus was inoculated mechanically to tobacco leaves，and the symptoms including mottle and mosaic leaves，could be observed in tobacco.Total RNA，extracted from infected pepper and tobacco with CTAB method，was used as template in RT-PCR with the special primers of pepper mild mottle virus (PMMoV).Expected fragments were obtained and sequenced.It showed that this virus was identified as PMMoV.RT-PCR detection system for PMMoV was established.

References:

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[2]王亚南,赵绪生,袁文龙,等.辣椒轻斑驳病毒研究现状[J].安徽农业科学,2010,38(14):7401-7402.

[3]Wang X，Liu F，Zhou G.Detection and molecular characterization of pepper mild mottle virus in China[J].Journal of Phytopathology,2005,154(11/12):755-757.

[4]Wang Y，Li X，Liu Y，et a1.Development of a simple and effective method for specific detection of Pepper mild mottle virus[J].Acta virologica,2009,53(1):21-27.

[5]Velasco L，Janssen D，Ruiz-Garcia L，et al.The complete nucleotide sequence and development of a differential detection assay for a pepper mild mottle virus (PMMoV) isolate that overcomes L3 resistance in pepper[J].J Virol Methods,2002,106:135-140.

[6]Ikegashira Y，Ohki T，Ichiki U T，et a1.An immunological system for the detection of pepper mild mottle virus in soil from green pepper fields[J].Plant Disease,2004,88(6):650-656.

[7]郭京泽,刘鹏,崔铁军,等.辣椒种子中辣椒轻斑驳病毒的检测[J].检验检疫科学,2007(6):32-33.

Memo

Memo:

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