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Molecular Cloning and Sequence Analysis of afp1 Gene in Momordica charantiap(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年24期
Page:
120-123
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Sequence Analysis of afp1 Gene in Momordica charantiap
Author(s):
ZHU Mei-xia; HAN Gai-ying; WANG Lan-ming; MA Xing-shu
College of Agriculture，Hebei University of Engineering，Handan，Hebei 056038
Keywords:
Momordica charantiap; afp1; RT-PCR; clone; sequene analysis
PACS:
Q 943.2
DOI:
-
Abstract:
Total RNA of Momordica charantiap was extracted using guanidine isothiocyantehod method.afp1 gene（394 bp）of Momordica charantiap was obtained by a step of RT-PCR method.afp1sequence was analyzed using DNA star software，the results showed afp1 sequence contained complete open reading frame.Identity percent of it with defensin gene of Brassica juncea was 87%.The results of the project would provide experimental evidence for molecular breeding of plants disease resistance.

References:

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Last Update: 2014-08-31