Study on Transferred Interleukin-4 Gene in Daucus carota L. by Cell Transformation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年24期

Page:

115-119

Research Field:

Publishing date:

Info

Title:

Study on Transferred Interleukin-4 Gene in Daucus carota L. by Cell Transformation

Author(s):

GAO Jin-qiu¹; 2; YU Li-jie¹; TAO Lei1; ³

1.College of Life Science and Technology，Harbin Normal University，Harbin，Heilongjiang 150025；

2.College of Life Sciences，Baicheng Normal University，Baicheng，Jilin 137000；

3.College of Life Sciences，Northeast Forestry University，Harbin，Heilongjiang 150040

Keywords:

Daucus carota L.(carrot); interleukin-4 gene(il-4 gene); genetic transformation; Agrobacterium tumfaciens

PACS:

Q 943.2；812

DOI:

-

Abstract:

Taking the hypocotyls of ‘Changchenghongyue’ and ‘Kaituozhe’ of Daucus carota L. as materials,the effect of the strains，the condition of pretreatment，concentration of Agrobacterium suspension，the time of infection and the time of co-cultivation on the transformation of carrot ‘Changchenghongyue’ and ‘Kaituozhe’ hypocotyls were studied.The results showed that the C58C1 strain was less damaged for carrot hypocotyls;and the transformation frequency was up to 60% for hypocotyls of ‘Changchenghongyue’，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 10 days，infected with suspension of A.tumefactions at the concentration of 3.2×107 cells/mL for 6 mins，co-cultivated for 3 days；and the transformation frequency was up to 100% for hypocotyls of ‘Kaituozhe’，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 3 days，infected with suspension of A.tumefactions at the concentration of 4.8×107 cells/mL for 10 min，co-cultivated for 4 days or derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 10 days，infected with suspension of A.tumefactions at the concentration of 3.2×107 cells/mL for 10 min，co-cultivated for 2 days;and the transformants were more for both of their hypocotyls，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 3 days，infected with suspension of A.tumefactions at the concentration of 1.6×107 cells/mL for 2 mins，co-cultivated for 2 days.

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