Anatomy of Lactarius akahatsu Tanaka and Selection of Culture Medium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Anatomy of Lactarius akahatsu Tanaka and Selection of Culture Medium

Author(s):: CHU Yang¹; NI Xin-jiang¹; JIANG Ming-yuan²; LIU Chuan-lin¹; WEN Shao-chao¹; SHAN Chang-you¹; 1.College of Life Science，Yantai University，Yantai，Shandong 264005；
2.Kunyushan Forest Farm of Yantai，Yantai，Shandong 264005

Keywords:: Lactarius akahatsu Tanaka; anatomy; tissue separation; selection of culture medium

Abstract:: The anatomy of Lactarius akahatsu Tanaka that were collected in field of Fushan area Yantai city were studied.The mycelia of Lactarius akahatsu were cultivated by the method of tissue separation and the culture medium of Lactarius akahatsu were selected.The results showed that the mycelium of Lactarius akahatsu was khaki.The mycelium had two caryon without clamp connection.The color of fruit-body was salmon pink.The stipe of it was growth in central pileus，without veil.The flesh of Lactarius akahatsu constituted by sphaerocyst and hyphae.The basidium of Lactarius akahatsu was strong，there were four basidiospore on the top of each basidium.The spore of Lactarius akahatsu was spherical，the drop of oil were big.The optimal of mother culture medium was improved MMN.On improved MMN mycelium growth rate was 1.17 mm/d,the mycelium was dense.The growth rate on PDA and cotton seed hull were 1.16 and 1.13 mm/d.The there culture medium had no significant difference in growth rate.

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Last Update: 2014-08-31