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Study on in vitro Culture and Plantlet Regeneration from Unpollinated Ovary of Cucumis sativus L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年23期
Page:
131-134
Research Field:
Publishing date:

Info

Title:
Study on in vitro Culture and Plantlet Regeneration from Unpollinated Ovary of Cucumis sativus L.
Author(s):
LI Jian-xin; GE Gui-min; PANG Shu-min; FANG Guan-na; WU Xiao-bo; ZHOU Hai-xia
Zhengzhou Vegetable Research Institute，Zhengzhou，Zhengzhou 450015
Keywords:
Cucumis sativus L.; unpollinated overies; embryoid; regenerated plant
PACS:
S 642.203.6
DOI:
-
Abstract:
Taking the unpollinated overies of them Henan Cucumis sativus L. as explant,the induction of embryoid and plant regeneration of three varieties of were studied.The results showed that regenerated plants were obtained in three varieties of cucumber.Different varieties，heat-treated time，concentration of TDZ and shape of explants affected the frequency of embryoid induction.Different varieties and concentration combinations of 6-BA and NAA affected the frequency of plant regeneration.Heat-treated for three days，sticks of explants and 0.06~0.08 mg/L of TDZ had high frequency of embryoid induction.0.05 mg/L of NAA and 0.4~0.6 mg/L of 6-BA could facilitate seedlings regeneration from embryoid.

References:

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[4]刁卫平,陈劲枫,雷春,等.影响黄瓜未授粉子房培养胚发生因素的研究[J].南京农业大学学报,2008,31(1):137-140.

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[6]徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学通报,2003,20(2):227-237.

[7]李建欣,庞淑敏,方贯娜,等.辣椒花药培养的胚状体分化及其与成苗率的关系[J].植物生理学通讯,2009(8):794-796.


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Last Update: 2014-08-31