Tissue Culture and Clone Construction of Crassula argentea Thunb(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年23期

Page:

125-128

Research Field:

Publishing date:

Info

Title:

Tissue Culture and Clone Construction of Crassula argentea Thunb

Author(s):

PU Shi-ming¹; 2; LI Rong-feng¹; FANG Li-juan¹; LAN Cui-ling¹; ZHOU Zu-ping²

1.Department of Chemistry and Life Science，Baise University，Baise，Guangxi 533000；

2.College of Life Science，Guangxi Normal University，Guilin，Guangxi 541004

Keywords:

Crassula argentea Thunb; callus; proliferation; adventitious bud; globular embryos

PACS:

S 681.903.6

DOI:

-

Abstract:

Taking Crassula argentea Thunb lamina as the explant and adding different concentrations of 6-BA and IBA in the culture medium，the influence of concentration of 6-BA and IBA on each stage in the Crassula argentea Thunb tissue culture forming process were studied.The results showed that MS+IBA 5 mg/L+6-BA 5 mg/L was the best medium for callus induction with unconspicuous differentiation and an inductivity of 95.56%；MS+IBA 2 mg/L+6-BA 1 mg/L was the best for adventitious bud induction directly with an inductivity of 86.67%，that could achieve more than 12 buds in average；MS+IBA 5 mg/L+6-BA 5 mg/L was the best for callus proliferation and the proliferation multiples was 10.9；the best for callus differentiation was MS+IBA 1 mg/L+6-BA 5 mg/L，the number of buds per unit area was more than 32 and the buds were obvious；when the medium was MS+IBA 2 mg/+6-BA 5 mg/L，the globular embryos produced more on the tissue surface with a long duration；the best for sound seeding was MS+IBA 2 mg/L，the average length of buds could be 4.74 cm after 45 d；on the medium with MS+IBA 0.5 mg/L，the inductivity of root inducing could be 100%.

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