Study on in vitro Culture and Plant Regeneration of Menyanthes trifoliata(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on in vitro Culture and Plant Regeneration of Menyanthes trifoliata

Author(s):: LI Wen-jie¹; PU Xiao-lan²; CHEN Ze-ying³; 1.Department of Forestry，Southwest Forestry University，Kunming，Yunnan 650224；
2.Department of Biological Science，Southwest Forestry University，Kunming，Yunnan 650224；
3.Department of Landscape Architecture，Southwest Forestry University，Kunming，Yunnan 650224

Abstract:: Taking the stems without axillary bud of Menyanthes trifoliate as explants，its in vitro culture and regeneration were studied.The results showed that putting the stem on the medium MS+6-BA 1.0 mg/L+IBA 0.5 mg/L for 10 d could produce callus，then put them on MS+6-BA 2.5 mg/L+NAA 0.2 mg/L+KT 0.05 mg/L for 15 days，it could differentiate into seedlings without root；taking the stems with bud on MS+IBA 0.1～0.2 mg/L for 7 days，axillary bud could germinate and then be seeding without root.Put the seedling on 1/2MS+IBA 0.5 mg/L for 3~5 d，it could rooting.

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Last Update: 2014-08-31