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Effect of the Filtered Solution of Colletotrichum gloeosporioides Penz.on the Growth and Differentiation of Callus in Lycium barbarum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年23期
Page:
112-115
Research Field:
Publishing date:

Info

Title:
Effect of the Filtered Solution of Colletotrichum gloeosporioides Penz.on the Growth and Differentiation of Callus in Lycium barbarum L.
Author(s):
QU Ling1; SHI Zhi-gang1; JIAO En-ning1; LI Yan-long1; ZHANG Zong-shan2; CAO You-long1
1.National Engineering Research Center of Wolfberry，Ningxia Academy of Agricultural and Foresty Sciences，Yinchuan，Ningxia 750002；
2.Institute of Plant Protection，Ningxia Academy of Agricultural and Foresty Sciences，Yinchuan，Ningxia 750002
Keywords:
Colletotrichum gloeosporioides Penz.; culture filtrate; callus; selection method and pressure; toxic effect
PACS:
S 436.639
DOI:
-
Abstract:
The effect of the filtered solution of Colletotrichum gloeosporioides Penz. on the growth and differentiation of calli in Lycium bararum L.were studied.The results indicated that the crude toxin had toxic effect and evidently inhibited the growth and differentiation of calli in Lycium barbarum L.in vitro.Compared with one-step screening system，the resistance of callus to Lycium barbarum L.toxin could be improved by increasing the concentration gradually in multi-step screening system.The toxin concentration of 50% could be considered as the selection pressure of resistant cell line and the concentration of 20% could be regarded as the screen concentration of differentiation of resistant cell line to Colletotrichum gloeosporioides Penz.

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Last Update: 2014-08-31