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Comparison on Effects of Different Methods on Extract Genomic DNA of Wild Litchi Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年23期
Page:
105-109
Research Field:
Publishing date:

Info

Title:
Comparison on Effects of Different Methods on Extract Genomic DNA of Wild Litchi Leaves
Author(s):
LIU Kai-dong; YUAN Chang-chun; CHEN Yan; MO Qiu-mei; LI Hai-li
School of Life Science and Technology，Zhanjiang Normal University，Zhanjiang，Guangdong 524048
Keywords:
wild litchi; genomic DNA; SDS method; CTAB method
PACS:
S 667.1
DOI:
-
Abstract:
Experiment was conducted with three different leaves of wild litchi from Guangdong and Guangxi province.The genomic DNA extraction of three methods namely SDS，CTAB and modified 2×CTAB were compared.The aim was to obtain the high quality DNA extracted method from leaves of wild litchi which contained plenty of secondary substances such as polysaccharide，polyphenols，pigment and protein.The results showed that the modified 2×CTAB method could extract the genomic DNA from wild litchi leaves of the highest purity and best quality.It also could apply on the PCR amplificative analysis on the cpDNA trnL-F gene of wild litchi leaves with distinct strips after amplification.Therefore，the modified 2×CTAB method was the better method to extract the genomic DNA from wild litchi leaves.

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Last Update: 2014-08-31