Effect of Medium Components on Pollen Germination in vitro of Diervilla sessilifolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effect of Medium Components on Pollen Germination in vitro of Diervilla sessilifolia

Author(s):: LIU Xue-lian; QIN Jia-mei; SUN Jun-bo; Department of Biology，Tonghua Normal University，Tonghua，Jilin 134002

Keywords:: Diervilla Sessilifolia; pollen germination; culture medium ingredient

Abstract:: Taking the brocade belt fresh pollen as material，using orthogonal design L₁₆(45),the effect of sucrose density，the calcium ion density，the boric acid density on the brocade belt pollen exsomatize were studied，to screen brocade belt pollen exsomatize sprouts the culture medium ingredient which was suitable.The results showed that the sucrose density and the calcium ion density showed large effect on the pollen germination and the growth influence，the boric acid influence was inferior that the two were remarkable.The brocade belt pollen exsomatize sprouts that were most suitable the culture medium ingredient was：sucrose 25%，boric acid 0.016%，calcium ion 0.0050%.

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Last Update: 2014-09-01