Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances

Author(s):: WENG Liang¹; WEN Lu²; 1.Department of Food Engineering，Jiangsu Food Science College，Huai’an，Jiangsu 223003;
2.Institute of Resource Microorganism，Huaiyin Normal University，Huai’an，Jiangsu 223300

Keywords:: Cordyceps militaris; Na₂SeO₃; CaCl₂; liquid shake culture; active substances

Abstract:: Adding different levels of Na₂SeO₃ and CaCl₂ to the culture medium，with the method of liquid shake culture，the effect of 2 trace elements on the content of cordycepin and adenosine，polysaccharide and cordyceps acid were studied，with HPLC to test the level of cordycepin and adenosine；with phenol-sulphate acid to test the level of polysaccharide；with sodium periodate colorimetric to test the level of cordyceps acid.The results showed that adding suitable selenium or calcium could obviously raised the content of Cordyceps militaris active substances.Adding 15 mg/L Na₂SeO₃ to the culture medium and the level of cordycepin and cordyceps acid had raised 29.0% and 34.9% respectively.Adding 16 mg/L CaCl₂ and the level of cordycepin had raised 24.05%.

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Last Update: 2014-08-31