Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.‘Hanfeng’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.‘Hanfeng’

Author(s):: LI Jin-ying; ZHAO Chun-li; ZHANG Zhi-dong; College of Horticulture，Jilin Agricultural University，Changchun，Jilin 130118

Abstract:: Taking different part of Ribes nigrum L.‘Hanfeng’ as materials,the factors that influencing culture in vitro of ‘Hanfeng’ were studied by in vitro culture of different parts of organs，including stem tip and stem with auxiliary bud.The results showed that the optimal sterilization time of stem tip was 7 minutes with 0.1% HgCl₂ and 9 minutes of auxiliary bud,tender stem with hand shelce had the better sterilize effect,0.1 HgCl₂ plus two drops of Twain 8 min had betler effect.The cluster buds could be induced in MS+BA 1.0 mg/L+IBA 0.1 mg/L+GA₃ 1.0~2.0 mg/L，and the plants grew well.

[1]张志东,李亚东,吴林.英国黑加仑的栽培与研究及我国黑加仑发展策略[J].吉林农业大学学报,2003,25(3):296-300.

[2]刘洪章,文连奎,郝瑞,等.黑穗醋栗果实营养成分研究[J].吉林农业大学学报,1998,20(3):1-4.

[3]赵锦,陈瑜,薛陈心,等.波兰黑穗醋栗的组织培养[J].河北林果研究,2006,21(4):419-421.

[4]滕树明,宋钟伍,齐凤莲,等.黑穗醋栗新品种-寒丰[J].中国果树,1997(3):6,10.

[5]宋钟伍,齐凤莲,周文志,等.寒丰黑穗醋栗的修剪技术[J].中国果树,2000(3):38.

[6]周文志.寒丰黑穗醋栗与薄皮黑穗醋栗主要性状比较试验[J].中国林副特产,2000,52(1):15.

[7]张彦妮,李博,安勇,等.婆婆纳组织培养再生体系的建立[J].东北林业大学学报,2010,38(12):46-18.

Last Update: 2014-08-30