|Table of Contents|

Study on DNA Extraction of Saintpaulia ionantha for AFLP Reaction System(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年22期
Page:
104-107
Research Field:
Publishing date:

Info

Title:
Study on DNA Extraction of Saintpaulia ionantha for AFLP Reaction System
Author(s):
SUN Yong-jian1; SUN Ning2; CAO Zhi-pan3; CHEN Xiao-qiang2; ZHANG Nai-nan2; ZHANG Lei2
1.Tianjin Tianshi College，Tianjin 301700；
2.Tianjin Agricultural University，Tianjin 300384;
3.Tianjin Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin 300020
Keywords:
DNA extraction; Saintpaulia ionantha; CTAB; freeze-drying; AFLP analysis
PACS:
S 681.2
DOI:
-
Abstract:
Taking the same leaves and stems of Saintpaulia ionantha as materials,fresh tissue extraction,drying tissue extraction,freeze drying tissue extraction,3 different pre-treatment were adapted,also DNA Kit,CTAB-Ⅰ,CTAB-Ⅱ 3 different extraction methods were adopt,to compare DNA fragment size,purity and yield throagh different extraction method.The best DNA extraction method for Saintpaulia ionantha which contained more polysaccharide polyphenols and moisture was determined.The results showed CTABⅡ method and the material processed by freeze-drying before test,namely,in the second extraction steps,added CTAB preeipitation in the method of DNA precipitation methed.DNA received by this method could be used as template for PCR in study at further molecular research.

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Last Update: 2014-08-30