Tissue Culture and Rapid Propagation of Lycoris chinensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: DAI Ping¹; ZHANG Chun-xia¹; ZHANG Zhi²; DENG Bo²; ZHOU Jian¹; 1.College of Nanjing Forestry University，Nanjing，Jiangsu 210037；
2.Jiangsu Junma Agricultural and Forestry Science and Technology Limited Company，Zhangjiagang，Jiangsu 215600

Abstract:: Seed mbryoes of Lycoris chinensis were used as explant in the study of tissue culture and rapid propagation.The results showed that the most suitable procedure for sterilization was to soak seeds into 0.1% HgCl₂ solution for 15 min after retreatment with 75% alcohol for 15 s.The results indicated that the best induced medium for callus induction was MS+2,4-D 2.0 mg/L.The best medium for bud differentiation was MS+6-BA 5.0 mg/L+NAA 0.5 mg/L.The best medium for little bulbs growth was MS+6-BA 3.0 mg/L+NAA 3.0 mg/L.The most optimum rooting medium was MS+NAA 1.0 mg/L，and the rooting rate was 81%.The rooting seedlings were effectively transplanted into base materials which contained nutritious soil and perlite (1∶1).The survival rate was up to 75%.

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Last Update: 2014-08-30