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Genetic Transformation Isoflavones Synthase Gene from Trifolium pratense Minshan Callus Mediated by Agrobacterium Tumefaciens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年21期
Page:
110-112
Research Field:
Publishing date:

Info

Title:
Genetic Transformation Isoflavones Synthase Gene from Trifolium pratense Minshan Callus Mediated by Agrobacterium Tumefaciens
Author(s):
HU Huan-huan; LI Wei-dong; JI Guo-jie; FENG Hui-gen
Department of Life Science and Technology,Xinxiang Medical University,Xinxiang,Henan 453000
Keywords:
Trifolium pratense Minshan; callus; agrobacterium tumefaciens; genetic transformation
PACS:
S 541+.2
DOI:
-
Abstract:
The callus was induced from the cotyledons and petioles of Trifolium pratense Minshan,and the callus was used for the receptor of genetic transformation mediated by agrobacterium tumefaeiens.The resistant callus was screened and confirmed to be transformants by PCR.The results showed that genetic transformation of Trifolium pratense Minshan callus mediated by Agrobaetenum tumefaeiens was entirely feasible.

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Last Update: 2014-09-01