The Callus Induction and Shoot Differentiation of Pedicels in Tissue Culture of Iris ensata L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: The Callus Induction and Shoot Differentiation of Pedicels in Tissue Culture of Iris ensata L.

Author(s):: WANG Wen-yuan¹; WANG Wen-he²; ZHOU Wen-qiang¹; WANG Dan¹; SHI Guo-xu³; XU Yu-feng³; 1.Shenyang Botany Garden，Shenyang，Liaoning 110163；
2.Beijing Agricultural College，Beijing 102206；
3.Shenyang Agriculture University，Shenyang,Liaoning 110866

Abstract:: Two species of Japanese Iris ensata L.were used as test materials to develop callus induction and shoot differentiation of pedicels.The results showed that flower stalks was the best explants when the length of flower bud was 2~5 cm，and disinfected with 0.1% HgCl₂ for 10 min were the most effective with the average contamination rate of 15.00% and survival rate of 75%.The optimal medium developing callus induction and shoot differentiation of pedicels was MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L NAA，the induction rate was 59.09% and differentiation rate was 50%.

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Last Update: 2014-09-01