|Table of Contents|

Establishment of the Optimized AFLP Analysis System for Pomegranate(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年21期
Page:
79-82
Research Field:
Publishing date:

Info

Title:
Establishment of the Optimized AFLP Analysis System for Pomegranate
Author(s):
ZHAO Li-hua
College of Agriculture Sciences，Xichang College，Xichang，Sichuan 615013
Keywords:
pomegranates; AFLP; system; optimized
PACS:
S 665.4
DOI:
-
Abstract:
Taking genome DNA of pomegranate ‘Fenhongmudan’ as material，AFLP reaction system was optimized from time of Mse I/EcoRⅠ restriction enzyme digest system，template DNA dilution multiple and program of pre-amplification，template DNA dilution multiple of selective amplification.The results indicated that 300 ng genomic DNA was digested completely with 6 hours，the products of ligation was not diluted；starting melting temperature was 94℃ for pre-amplification；the products of pre-amplification was diluted for 20 times.

References:

[1]张玉星.果树栽培学各论[M].1版.北京:中国农业出版社,2008:78-86.

[2]汪小飞.石榴品种分类研究[D].南京:南京林业大学,2007:20-49.

[3]Stover E，Mercure E W.The pomegranate：a new look at the fruit of paradise[J].Hort Science，2007,42(5):1088-1092.

[4]卢龙斗,刘素霞,邓传良,等.RAPD技术在石榴品种分类上的应用[J].果树学报,2007，24(5):634-639.

[5]Ghobadi S，Khoushkhoui M，Tabatabaei B E S.Phylogenetic relationships among some iranin ponegranate accessions revealed by Inter-simple Sequence Repeat(ISSR) markers[J].Iranlan Journal of Horticultural Science and Technoogy Fall,2005，6(3):111-120.

[6]Narzary D，Rana T S，Ranade S A.Genetic diversity in inter-simple sequence repeat profiles across natural populations of Indian pomegranate(Punica granatum L.)[J].Plant Biology,2010,12(5):806-813.

[7]曹仪植.植物分子生物学[M].1版.北京:教育出版社,2004:142-159.

[8]周延清.DNA分子标记技术在植物研究中的应用[M].1版.北京:化学工业出版社,2005:2-179.

[9]赵丽华,王先磊.成熟石榴叶片DNA提取方法研究[J].安徽农业科学,2009,37(31):15141-15143.

[10]翟文慧,贾春枫,周莹,等.十字花科蔬菜黑腐病菌(Xanthomonas campestris?cv. campestris)AFLP分析体系的建立及优化[J].中国农学通报,2011,27(22):168-172.

[11]Aitken K S，Li J C，Jackson P，et al.AFLP analysis of genetic diversity within saccharum officinarum and comparison with sugarcane cultivars[J].Aust J Agric Res,2006，57：1167-1184.

[12]Li W，Xing S Y，Yang K Q，et al.Genetic relationships of ornamental cultivars of Ginkgo biloba analyzed by AFLP techniques[J].Acta Genetica Sinica,2006,33(11)：1020-1026.

[13]Byun S Q，Fang Q，Zhou H，et al.An effective method for silver-staining DNA in large numbers of polyacrylamide gels[J].Anal Biochem，2009，38(5)：174-175.

[14]Yu X Y，Li M，Liu Z，et al.A novel gene，screened by cDNA-AFLP approach，contributes to lowering the acidity of fruit in apple[J].Plant Physiology and Biochemistry，2007，45:139-145.

[15]张正银,刘波洋,陈放,等.麻疯树AFLP体系的建立与优化[J].应用与环境生物学报,2009,15(2):280-283.

[16]臧德奎,陈红,郑林,等.木瓜属优良品种亲缘关系的AFLP分析[J].林业科学,2009,45(8):39-43.

[17]赵志常,胡桂兵,刘运春,等.番荔枝DNA的提取和AFLP体系的建立[J].北方园艺,2009(10):44-47.

[18]明军,张启翔,晏小兰,等.梅花基因组AFLP银染反应体系的建立和优化[J].北京林业大学学报,2003,25(3):17-21.

[19]田晓俊,温强,汪信东,等.闽楠、红楠AFLP反应体系建立[J].林业科技开发,2009,23(3):38-42.

[20]欧立军,黄园,王俞人,等.天门冬AFLP反应体系的建立及优化[J].中国农学通报,2011,27(8):87-90.

[21]Yan Z，Denneboom C，Hattendorf A.Construction of an integrated map of rose with AFLP,SSR,PK,RGA,RFLP,SCAR and morphological markers[J].Theor Appl Genet,2005,110:766-777.

[22]刘欢.燕麦AFLP标记的遗传多样性分析[D].兰州:甘肃农业大学,2007.

[23]李元军,唐美玲,于青,等.富士苹果AFLP体系的优化及其在鉴定早熟芽变中的应用[J].园艺学报,2009,36(3):327-332.


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Last Update: 2014-09-01