rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge

Author(s):: LIU Ling-di; XIE Xiao-liang; WEN Chun-xiu; TIAN Wei; LIU Ming; GAO Xue-fei; Institute of Economic Crop Research，Hebei Academy of Agriculture and Forestry Sciences，Shijiazhuang,Hebei 050051

Keywords:: Salvia miltiorrhiza Bge; different germplasm; rDNA ITS sequence

Abstract:: In order to study rDNA ITS sequence of Salvia，the rDNA ITS sequence of twenty-eight Salvia germplasm from different area were analyzed.The results showed that the length of rDNA ITS gene sequence of Salvia was 600~619 bp，including ITS1 (227~228 bp)，5.8S (166 bp) and ITS2 (206~224 bp).There were thirty-one variable sites in the ITS1 area (it was 13.5% of total site)，twenty-five variable sites in the ITS2 area (it was 11.2% of total site).The ITS1 and ITS2 gene fragments were conservative at specific level in Salvia，while there were less difference among some germplasm，so they could be molecular marker for authentication of Salvia.

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Last Update: 2014-08-31