|Table of Contents|

Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年20期
Page:
117-119
Research Field:
Publishing date:

Info

Title:
Study on Cryo-preservation Technology with Vitrification of ‘Tongzi No.1’ Strawberry
Author(s):
XU Qi-hong; REN Ping-guo
Luohe Vocational Technology College，Luohe,Henan 462000
Keywords:
strawberry; cryopreservation; vitrification
PACS:
S 668.409+.3
DOI:
-
Abstract:
Researching on the ‘Tongzi No.1’strawberry meristem-tip cryo-preservation with vitrification，the effect of various factors of vitrification，such as，low temperature acclimatization time，pre-culture time，pretreatment time，PVS2 treatment time，were studied on survival rate of strawberry meristem-tip after cryo-preservation.The results showed that after 30 d and acclimatizing strawberry meristem-tips at 4℃，they were cultured in solid preculture medium after 4 d；pretreat for 30 min，suck up the pre-culture medium，and then put them into PVS2 at 0℃ for 80 min；then put them in the fresh PVS2，pre-serve in LN for 1 h；and then throw them rapidly into water at 40℃ for 1 min；wash the shoot tips 3 times at room temperature and then plant the tips to regeneration medium.The survival rate could reach the highest about 43% and rege-nerative plants become divided regularly.

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Last Update: 2014-08-31