The effects of different dark treatments and plant growth regulators combination on browning and rooting were studied respectively.The results showed that during the course of initial culture,sprout was cultured under dark for 7 d,and then sprout was cultured under normal light condition,and browning was relieved obviously.The most optimum medium for root primordium was 1/2MS+NAA 2.0 mg/L+IAA 2.0 mg/L+6-BA 0.1 mg/L or 1/2MS+NAA 1.0 mg/L+IBA 1.0 mg/L+AC 0.2 g/L.