Study on the Loose Callus Induction and Cell Suspension Culture of Lonicera japonica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Loose Callus Induction and Cell Suspension Culture of Lonicera japonica

Author(s):: WU Li-na; Department of Biology and Chemistry，Xingtai College，Xingtai,Hebei 054001

Abstract:: Taking ‘Juhua No.1’ as the material,the effects of explant,medium on the loose callus induction of L.japonica were investigated，as well as the effects of primary inoculums size and sucrose concentration in the medium on the growth of suspension culture cell were studied.The results showed that the best explant which induced the callus was the young leave and its optimal medium was MS+2.0 mg/L 2,4-D+0.5 mg/L KT.The best primary inoculums size of callus was 60 g/L and the cell of L.japonica growed best when sucrose concentration was 35 g/L in suspension culture.

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Last Update: 2014-08-31