Study on Tissue Cultivation Technique in the Hemerocallis hybrida ‘Shaman’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Cultivation Technique in the Hemerocallis hybrida ‘Shaman’

Author(s):: YANG Li-li¹; ZHANG Xiao²; YANG Rui¹; ZHANG Yan-qin¹; 1.Research Centre of Arid Farming，Shanxi Academy of Agricultural Science，Taiyuan，Shanxi 030006;
2.Second Middle School in Xiangyuan County，Xiangyuan,Shanxi 046200

Abstract:: Using ovary of Hemerocallis hybrida ‘Shaman’ as explant，callus induction and differentiation,the effect of hormone combinations on reproductive efficiency of plantlets,setting of rooting medium,the key technical parameters of domestication and cultivation in the process of its tissue culture were systematically studied.The results showed that the best culture medium of ‘Shaman’ callus differentiation was MS+4 mg/L 6-BA+0.3 mg/L IBA+1 mg/L 2.4-D and adventitious bud differentiation rate reached 49.2%.When 6-BA concentration was 3 mg/L and (0.3~0.5)mg/L IBA，propagation coefficient would be possible to reach 5.8~6.4 times.The suitable rooting medium included 1/2 MS+0.4 mg/L NAA+20 g/L sucrose+6.5 g/L agar，the average rooting number reached 5.7 roots.The substrate for transitional cultivation of tissue culture seedling was thick riversand，vermiculite+peat soil，peat soil，when the temperature was 24~28℃，the relative humidity was 85%~90%，survival rate of tube seeding reached 90%.

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Last Update: 2014-09-09