Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves

Author(s):: CHEN Guo-liang; CHEN Zong-li; SUN Ping; YANG Qing-hu; LIU Mi-mi; College of Life Science,Yan’an University，Shaanxi Key Laboratory of Chinese Jujube，Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources，Yan’an，Shaanxi 716000

Keywords:: young jujube leaves; total RNA; extraction method; electrophoresis

Abstract:: Taking young jujube leaves as material，RNA in jujube leaves was extracted by the DEPC water method，SDS method，CTAB-LiCl method and isothiocyanate guanidine method.The quality of the total RNA was tested by nuclear acid/protein analyzer，denaturation agarose gel electrophoresis and RT-PCR.The results showed that total RNA extracted by DEPC water method had the best quality and reliability，followed by SDS extraction method and CTAB-LiCl method，the isothiocyanate guanidine method was the worst.

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Last Update: 2014-09-09