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Study on Agrobacterium-mediated Transformation of Vitis vinifera (L.) and Plant Regeneration(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年19期
Page:
113-117
Research Field:
Publishing date:

Info

Title:
Study on Agrobacterium-mediated Transformation of Vitis vinifera (L.) and Plant Regeneration
Author(s):
TAN Chao; ZHANG Jian-xia; WANG Yue-jin
Key Laboratory of Melecular Biology of Shaanxi Province,Key Opening Laboratory of Plant Germplasm Resources,Northwest Gardening Plants of Ministry of Agricultural,College of Horticulture,Northwest Agricultural and Forestry University,Yangling,Shaanxi 712100
Keywords:
Vitis vinifera; floral organ; somatic embryo; plant regeneration; genetic transformation
PACS:
S 663.103.6
DOI:
-
Abstract:
Stamen，pistils and flower buds from Vitis vinifera cvs.‘Thompson Seedless’，‘Carignane’ and ‘Red Globe’ were used as explants to investigate their efficience of embryonic callus induction and Agrobacterium-mediated stilbene synthase gene transformation.The results indicated that MC medium was optimum for embryonic callus induction from stamens among 5 medium for embryonic callus induction.The induction rate in ‘Thompson Seedless’，‘Carignane’ and ‘Red Globe’ were 1.7% 0.3% and 11.8%，respectively.Embryonic callus from ‘Thompson Seedless’ and ‘Carignane’ could be induced in PIV medium with 4.0% and 3.2% induction rates.However，the highest induction rate occurred in embryonic callus from flower buds of ‘Red Globe’ in MC medium with 5.6% induction rate.Only flower buds from Redglobe could be induced in MS1 medium with 0.5% induction rate.A modified X6 liquid medium was suitable for secondary embryogenesis，and medium B favored germination and seedling of somatic embryos.Transformed plants were selected through resistance screening and 2 resistent plants of ‘Thompson Seedless’ were obtained.Results from PCR and PCR-Southern indicated that stilbene synthase gene were integrated into the genome of ‘Thompson Seedless’.

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Last Update: 2014-09-09