With the slag,corn straw,fermented cow dung,mushroom production waste,biogas residues,furfural residue as materials,experiment of substrate formulations on organic ecological soilless cultivation in pepper were studied.The results showed that substrate formulations which was 0.6∶0.6∶1∶1∶0.6 with slag,corn straw,cow dung,mushroom sticks,furfural in volume was fit to organic ecological soilless cultivation in pepper.