Research on Tissue Culture of Chosenia arbutifolia(Pall) A.Skv.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: REN Zhen; Shenyang Research Academy of Landscape Architecture Science,Shenyang,Liaoning 110016

Keywords:: Chosenia arbutifolia (Pall) A.Skv.; tissue culture; axillary bud

Abstract:: Taking the dormancy stem section as explant,the effect of sterilization time,plant hormone and cultivation on rooting and trasplanting of Chosenia arbutifolia(Pall) A.Skv. were studied.The results showed that sterilization time was best with 0.1%HgCl₂ in 8 min,in the subculture stage，shoots induction rate and multiplication rate were the highest，96.3% and (28.2±2.8)%，respectively on the medium MS+BA 1.00 mg/L+NAA 0.03 mg/L.The shoots roots well on the basic medium 1/2MS+IBA 0.5 mg/L and the rooting rate was 98.5%.In domestication，it was better to use vermiculite and leaf mold for the growth of tender shoots in the transplanting soils.

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Last Update: 2014-08-30