Study on the Technique of Tissue Culture and Rapid Propagation of Aristolochia contorta Bunge(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Technique of Tissue Culture and Rapid Propagation of Aristolochia contorta Bunge

Author(s):: SHAO Hong; SUN Rui; ZHANG Li-min; ZHANG Wei-dong; WANG Zhen; College of Life Science，Jiamusi University，Jiamusi,Heilongjiang 154007

Keywords:: Aristolochia contorta Bunge; tissue culture; callus; clumpy bud

Abstract:: Aristolochia contorta Bunge was used as materials for rapid propagated by plant tissue culture technique，and the plant regeneration system was established.The results showed that the optimal explants for callus induction were leaves，the medium of MS+IBA 0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L for callus induction was better；the optimal medium for differentiation of clumpy bud was MS+IBA 0.4 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L；the better medium for root induction was MS+IBA 0.4 mg/L+NAA 0.2 mg/L+6-BA 2.0 mg/L+KT 0.5 mg/L.

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Last Update: 2014-08-29