Zoysia japonica seeds during storage under natural，its life was rapidly decreased，the flow of trade to produce extremely serious effect.With the seeds of Zoysia japonica as test material，a series of different water gradients of Zoysia japonica seeds were used，the standard method (high temperature drying method) corrected gravimetric method for determination of high temperature over drying method correcting weight determination of gravimetric method were adopted，Zoysia japonicaseed moisture balance effect were studied.The results showed that high temperature drying method correcting weight determination，determination of Zoysia japonica seed moisture change，get the gravimetric method for determination of the correction equation was y=0.973x-0.033，R2=0.998，through the establishment of this equation，could quickly by gravimetric method estimating the water content in the range of 8.0%~21.5% Zoysia japonica seed moisture content，and did not damage the seed samples.