Study on the Optimal Method of Tissue Culture on Different Species of Wild Actinidia arguta(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Optimal Method of Tissue Culture on Different Species of Wild Actinidia arguta

Author(s):: LIU Yan-ji¹; HAO Gui-jie¹; JIANG Yan-yan²; TIAN Xiao-yan³; 1.College of Bioscience and Technology,Shenyang Agricultural University,Shenyang,Liaoning 110866；
2.College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866；
3.College of Food Science，Shenyang Agricultural University，Shenyang，Liaoning 110866

Keywords:: wild Actinidia argula; tissue culture; sprouting percentage; coeffient of multiplication

Abstract:: The tissue culture system about different species of wild Actinidia arguta (H1、H2、H3、H4 and H5) were established by using their stem segments explants.The best medium was studied and screened，which suited for different kinds of wild Actinidia arguta rapid propagation by the combination of 6-BA and NAA.The results indicated that the species and mediums had significantly impact on the stem segments explants.The most suitable medium for propagation in species H1 was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，the most suitable medium for propagation in species H2 was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L；the most suitable medium for propagation in species H3 and H4 was MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,the most suitable medium for propagation in species H5 was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L.

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Last Update: 2014-08-29