Silico Cloning and Sequence Analysis of F3H Gene of Chinese Cabbage(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年17期

Page:

116-118

Research Field:

Publishing date:

Info

Title:

Silico Cloning and Sequence Analysis of F3H Gene of Chinese Cabbage

Author(s):

MA Guang¹; LIANG Fei-fei¹; GUO Ji-ping¹; QI Shan-hou²; ZHONG Lu¹

1.Department of Life Sciences，Hengshui College，Hengshui，Hebei 053000；

2.Center for Disease Control and Prevention，Hengshui College，Hengshui，Hebei 053000

Keywords:

Flavanone 3-hydroxylase; Chinese cabbage; in silico cloning

PACS:

S 682.36

DOI:

-

Abstract:

Flavanone 3-hydroxylase (F3H)，a critical enzyme，catalyzes an step in anthocyanin biosynthesis.According to the conservation of homologous gene，with F3H gene in Brassica napus,the cDNA of F3H gene in Chinese cabbage was in silico cloned.Some characters of the F3H，such as the composition of amino acid sequences，physical and chemical properties，hydrophobicity or hydrophilicity，secondary and tertiary structure of the protein and function were analyzed and predicted by the tools of bioinformatics.The results showed that the length of cloned F3H cDNA was 1 228 bp and it contained a complete ORF (1 077 bp)，encoded 358 amino acids.The calculated molecular weight of it was 40 104.6 Da，theoretical pI of 5.45.It was a bulbous structure with 7 helices，12 sheets，and some irregular coils in tertiary structure.

References:

[1]Forkmann G，Heller W，Grisebach H.Anthocyanin biosynthesis in flowers of Matthiola incana.Flavanone 3-and flavonoid 3′-hydroxylases[J].Z Naturforsch Sect C Biosci，1980，35:691-695.

[2]Britsch L，Ruhnau-Brich B，Forkmann G.Molecular cloning，sequence analysis，and in vitro expression of flavanone 3 beta-hydroxylase from Petunia?hybrida[J].J Biol Chem,1992，267:5380-5387.

[3]Shen G A，Pang Y Z，Wu W S，et al.Cloning and Characterization of a Flavanone 3-Hydroxylase Gene from Ginkgo biloba[J].Biosci Rep，2006，26:19-29.

[4]Xu B B，Li J N，Zhang X K，et al.Cloning and molecular characterization of afunctional flavonoid 3′-hydroxylase gene from Brassica napus[J].J Plant Physiol,2007，164：350-363.

[5]马光.芜菁花青素合成关键基因F3H启动子区序列及蓝光受体CRY1基因的克隆与初步功能分析[D].哈尔滨:东北林业大学，2008.

[6]Zhang H M，Jiang M G，Feng Y J.In silico cloning of MgEno-1 cDNA from Magnaporthe grisea[J].China J Bioinformatics，2005(4)：57-61.

Memo

Memo:

-

Common functions