Biological Characteristic of Leaf Blight：A New Cycad Disease in Guizhou Province(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Biological Characteristic of Leaf Blight：A New Cycad Disease in Guizhou Province

Author(s):: XIAO Zhong-jiu; LI Xiao-xia; XU Ai-bin; LUO Hua-yan; PAN He-jun; Department of Biology，Zunyi Normal College，Zunyi，Guizhou 563002

Abstract:: This paper aimed to study the biological characteristics of the pathogen (Phomopsis cycadis)causing leaf blight on cycad in Guizhou province，the effects of temperature，pH，illumination，the sources of nitrogen and carbon on the mycelium growth and the germination of conidiospore of the pathogen were explored，the study of this paper systematically made a research on the biological characteristics of Phomopsis cycadis，it could provide a theoretical basis for the prevention and control of the cycad disease.The temperature range was 10~35℃ for the mycelia growth and 15~35℃ for the conidiospore germination of the pathogen，the optimal temperature for mycelia growth and conidiospore germination were 30℃ and 25℃.The pathogen could grow and conidiospore germination with a pH of 2~12，while the optimal pH was 6.0 for mycelia growth and 10 for conidiospore germination.Light hardly affected the growth of fungus mycelium and the conidiospore germination.The pathogen could use monosaccharide，disaccharide，polysaccharide，the best mycelia growth and conidiospore germination were observed with sucrose as the carbon source，and the pathogen could use organic and inorganic nitrogen，the best mycelia growth were observed with KNO₃ as the nitrogen source，but not suitable for conidiospore germination.

[1]Kowalskarnt,Itzhak y,Puett D.Presence of aromatase inhibitors in cycads[J].JEthnopharmacol，1995，47(3)：113-116.

[2]江苏新医学院.中药大词典[M].上海：上海科学技术出版社，1979：491-49.

[3]孔繁翠，顾昊，王鹤尧.铁树提取物对人肺腺癌细胞凋亡的诱导及相关机制的初步研究 [J].中国新药杂志，2008，17（8）：667-672.

[4]肖龙骞，朱华.苏铁nrDNAITS区的序列多态性：不完全致同进化的证据[J].生物多样性，2009，17(5)：476-481.

[5]Baker C A，Adkins S.Tobacco ringspot virus Found in the Cardboard Cycad (Zamia furfuracea) in Florida[J].Plant Disease，2007，91(1)：112.

[6]王新荣，李楠，林月娥.苏铁镰刀菌球茎腐烂病化学防治技术研究[J].中国森林病虫，2005，24（6）：34-37.

[7]方中达．植病研究方法[M]．北京：中国农业出版社，1998：123-125,152-153．

[8]陆家云．植物病原真菌学[M]．北京：中国农业出版社，2001：55-56．

[9]董国菊，申晚霞.重庆地区玉米圆斑病菌生物学特性的测定[J].西南大学学报(自然科学版)，2010，32（12）：8-13.

[10]李小霞，肖仲久，李黛.草乌白绢病菌生物学特性研究[J].广东农业科学，2010(8)：128-130.

[11]卢东升，王明好，贾晓.野菊内生真菌生物多样性与生态分布[J].东北林业大学学报，2011，39（8）：88-89.

[12]刘超，师俊玲，周小娟，等.产PDG杜仲内生菌的分离筛选和分类鉴定及生长条件研究[J].西北农林科技大学学报(自然科学版),2011，39（1）：203-209.

[13]王玉美，陈洪章.产喜树碱内生真菌的筛选及鉴定[J].微生物学通报2011，38(6)：884-888.

[14]姜淑霞，马洪兵.板栗上的拟茎点霉一新种[J].菌物学报，2010，29(4)：467-471.

[15]郑华英，钱国良，徐福元，等.苏北杨树新造林地溃疡病病原菌的鉴定[J].林业科学研究，2010，23(5)：649-655.

Last Update: 2014-08-28