The RAPD Analysis of Papaver nudicarule in Shanxi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: KANG Xiu-ping; CHANG Xiao-xiao; CHEN Lin; LI Sen; XING Guo-ming; College of Horticulture，Shanxi Agricultural University，Taigu,Shanxi 030801

Abstract:: To provide bases of establishing Papaver nudicarule genetic relationship and genetic diverslty,taking 7 Papaver nudicarule collected and preserved from Shanxi and Inner Mongolia as test materials， DNA was extracted by improved CTAB and concentration and dosage of template,dNTP,Mg²⁺,Taq DNA polymerase,dNTP and primer were screened by single-factor multi-level gradient experiment.The experiment established RAPD technology amplification system on Papaver nudicarule in Shanxi Province.The results showed that the optimized RAPD system was Taq DNA polymerase 2.0 U,template DNA 20 ng,primer 1.2 μmol/L,Mg²⁺ 2.0 mmol/L and dNTP 150 μmol/L in total volume 20 μL amplification system and on this basis builded UMPGA dendrogram.Clustering results showed that geographical distribution of Papaver nudicarule was the leading factor,and the flower color was a secondary element.

[1]庄漩.婴粟科植物的分类、进化与分布[J].云南植物研究,1993,15(2):137-148.

[2]李云祥,甄占萱,马双马,等.野罂粟生态调查及引种驯化研究[J].安徽农业科学,2007,35(19):5834-5835.

[3]冯夏莲,何乘忠,张志毅,等.植物遗传多样性研究方法概述[J].南林学院学报,2006(26):69-79.

[4]刘龙昌,向其柏,刘玉莲.RAPD标记在桂花遗传多样性检测和品种鉴定中的应用(英文)[J].南京林业大学学报(自然科学版),2004,28(zl):76-82.

[5]伊艳杰,黄莹,尚富德.利用RAPD研究桂林桂花品种间的亲缘关系[J].广西植物,2005,25(2):129-133.

[6]谢伟,乐超银,周艳.5种中国兰花RAPD反应条件的优化[J].热带农业科学,2005,25(4):19-23.

[7]戴思兰,陈俊愉,李文彬.菊花起源的RAPD分析[J].植物学报,1998,40(11):1053-1059.

[8]王小全,邹喻苹,张大明,等.PAPD应用于遗传多样性和系统学研究中的问题[J].植物学报,1996,38(12):954-962.

[9]刘春林,官春云,李栒.关于植物随机扩增多态性的DNA的可靠性问题[J].植物生理学通讯,2000,36(1):53-59.

[10]裴严龙,邹喻苹,尹秦,等.矮牡丹与紫斑牡丹RAPD分析初报[J].植物分类学报,1999,33(4):350-356.

Last Update: 2014-08-28