Study on Key Technology of Production Tissue Culture Seedling of Enstoma grandiflorun(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Key Technology of Production Tissue Culture Seedling of Enstoma grandiflorun

Author(s):: ZHONG Bo; Seed Administration Station of Guangxi Zhuang Autonomous Region，Nanning，Guangxi 530022

Abstract:: Taking the capsuled of Enstoma grandiflorun with pink flower as the material,the key technologies of production tissue culture seedling of Enstoma grandiflorun were studied.The mediums of leaves induction，single bubs enrichment culture and rooting were elected and the other methods of production were summarized.The results showed that the best medium of leaves induction was MS+6-BA 0.4 mg/L+IBA 0.1 mg/L and the normal bubs every leaf was 5 in it.The best medium of single bubs enrichment was MS+6-BA 0.2 mg/L+IBA 0.05 mg/L and the normal bubs every single bubs was 5 in it.The best medium of rooting was 1/2MS+IBA 1.0 mg/L and rooting rate was 95% in it.

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Last Update: 2014-08-28