Study on Isolated Microspore Culture and Plant Regeneration of Carrot(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年15期

Page:

126-129

Research Field:

Publishing date:

Info

Title:

Study on Isolated Microspore Culture and Plant Regeneration of Carrot

Author(s):

YIN Li-rong¹; GUAN Chang-zhi¹; CHEN Lei¹; FU Ren-sheng¹; HUO Wen-juan²; ZHAO Heng²

1.Tianjin Agricultural Biotechnique Center,Tianjin 300384;

2.Tianjin Academy of Agricultural Science,Tianjin 300192

Keywords:

carrot; isolated microspore culture; plant regeneration; double haploid

PACS:

S 631.203.6

DOI:

-

Abstract:

The culture of isolated microspore with 2 carrot cultivars‘Changchenghongyue’，‘Baoguan’ were studied.Stage for simpling，metheds for microspore isolating and effective sterilizing were fixed.The results indicated that difficult differences were existed between two genotypes in isolated microspore culture，haploid and dihaploid were atained by inducing embryo calli，regeneration and root formation，the ratio of haploid regenerate plant was 46.9%,double haploid was 53.1%.It also showed that the most effective culture media for inducing embryo calli，regenerate plant and root formation were HLB+0.5 mg/L 2,4-D，MS+0.5 mg/L 6-BA and MS（or W14）+1.0 mg/L NAA。

References:

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