|Table of Contents|

Comparison of Three Optimized Method for Isolating Total RNA from Grapevine Tissues(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年15期
Page:
119-122
Research Field:
Publishing date:

Info

Title:
Comparison of Three Optimized Method for Isolating Total RNA from Grapevine Tissues
Author(s):
SONG Yang-bo; LIU Yan-lin; GENG Wan-gang
College of Enology，Northwest Agricultural and Forestry University，Yangling Shaanxi 712100
Keywords:
grapevine; total RNA; isolation; optimized SDS-phenol method; optimized CTAB method; optimized Trizol method
PACS:
S 663.1
DOI:
-
Abstract:
In order to choose the best optimized method for isolating total RNA from grapevine tissues，the effects of optimized SDS-phenol method，optimized CTAB method and optimized Trizol method were compared by isolating total RNA from the grapevine buds，leaves and ripe berry skins.The results showed that quality and high cencentrition RNA were harvested by the optimized SDS-phenol method and the optimized CTAB method and the two methods are more suitable for RNA isolation from leaves and buds.At the same time，quality but less cencentrition RNA were gotten by the optimized Trizol method，while the total RNA was still enough for further research.

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Last Update: 2014-08-28