Determination of Ellagic Acid in Strawberry by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年15期

Page:

25-27

Research Field:

Publishing date:

Info

Title:

Determination of Ellagic Acid in Strawberry by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography

Author(s):

YANG Yuan¹; SHI Lei¹; ZHANG Jia¹; LI Wen-sheng¹; ZHANG Kai-chun¹; FENG Xiao-yuan¹; 2; WANG Bao-gang¹

1.Institute of Forestry and Pomology，Beijing Academy of Agricultural and Forestry Sciences，Beijing 100093;

2.Beijing Research Center for Agrifood Testing and Farmland Monitoring，Beijing 100097

Keywords:

DSPE; HPLC; ellagic acid; strawberry

PACS:

Q 946

DOI:

-

Abstract:

An analytical method was developed to extract and determine content of free ellagic acid in strawberry using dispersive solid phase extraction and high performance liquid chromatography (DSPE-HPLC).The free ellagic acid in strawberry was extracted under 0.1% trifloroacetric acid-methane,and purified by C₁₈ dispersive solid-phase extraction.Then strawberry sample was separated by column Poroshell 120 SB-C₁₈ (100 mm×4.6 mm，2.7 μm) and measured at UV wavelength of 254 nm with the mobile phase consisting of acetonitrile∶water (0.1% trifloroacetric acid) (15∶85，V/V).The method could determine the free ellagic acid in strawberry and had fine linear relationship (r=0.99981) when the range of ellagic acid was within 0.1~20 μg/mL.Besides that，the minium detection concentration was 0.0134 μg/g，and average recoveries were 85.3%~95.1% with relative standard deviation (RSD，%)<10%.In summary，this approach using DSPE-HPLC was capable to determine free ellagic acid in strawberry.

References:

[1]Angelika R，Ronald E W.Ellagic acid content of red raspberry juice as influenced by cultivar，processing，and environmental factors [J].J Agric Food Chem,1993,41:1951-1960.

[2]李小萍,梁琪,辛秀兰,等.红树莓果中鞣花酸提取物的抗氧化性研究[J].食品科技,2010,35(5):182-185.

[3]陆晶晶,丁柯,杨大进.保健品功能因子鞣花酸研究进展[J].食品科学,2010,31(21):451-454.

[4]李小萍,梁琪,辛秀兰,等.高效液相色谱法测定红树莓中鞣花酸含量[J].安徽农业科学,2009,37(24):11345-11346.

[5]张强.用溶剂法提纯工业单宁酸工艺的研究[J].西北大学学报(自然科学版),1997,27(4):313-315.

[6]陈笳鸿,吴冬梅,汪咏梅,等.紫外分光光度法测定鞣花酸含量的研究[J].生物质化学工程,2007,41(3):18-20.

[7]Lin T Y，Vine R P.Identification and reduction of ellagic acid in muscadine grape juice[J].J.of Food Science,1990,55(6):1607-1609.

[8]周本宏,吴振华,李小军,等.高效毛细管电泳法测定石榴皮中鞣花酸的含量[J].中国药方,2005,16(24):1893-1894.

[9]刘艳,宋立秋,范俊娟,等.HPLC法测定蓝莓中鞣花酸含量[J].江苏农业科学,2008(3):217-219.

[10]周本宏,吴振华,刘春,等.高效液相色谱测定石榴皮鞣花酸的含量[J].广东药学院学报,2005,21(6):693-694.

[11]晋艳曦.葡萄汁中鞣花酸的定量与定性分析[J].饮料工业,1999,2(5):33-36.

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