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Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年13期
Page:
185-187
Research Field:
Publishing date:

Info

Title:
Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.
Author(s):
WEI Kun-hua; LI Lin-xuan; WANG Mei-ying; ZHANG Zhan-jiang; MIAO Jian-hua
Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Guangxi Botanical Garden of Medicinal Plants，Nanning，Guangxi 530023
Keywords:
Sophora tonkinensis Gapnep.; matrine; oxymatrine; tissue-cultured plantlet
PACS:
S 567.1+9
DOI:
-
Abstract:
With Sophora tonkinensis Gapnep tissue culture seedling as material,the presence of matrine and oxymatrine in the tissue-cultured plantlets of Sophora tonkinensis Gapnep.were identified by TLC，and also the matrine and oxymatrine contents of tissue-cultured plantlets before and after transplanted were determinated by HPLC.The results showed that there were reddish orange spots at the corresponding place of matrine and oxymatrine.The analysis of HPLC showed that both of the tissue-cultured plantlets before transplanted and after transplanted in the field for four monthes possessed matrine and oxymatrine，but lower than the seedlings from seeds planted in the field for half year.This study demonstrates that the tissue-cultured plantlets of S.tonkinensis Gapnep.contain matrine and oxymatrine，and it will provide a good support for follow-up research.

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Last Update: 2014-09-17