|Table of Contents|

CAPS Marker and Detection of Resistant Gene Tomato Yellow Leaf Curl Virus Disease Ty-1 to Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年13期
Page:
136-139
Research Field:
Publishing date:

Info

Title:
CAPS Marker and Detection of Resistant Gene Tomato Yellow Leaf Curl Virus Disease Ty-1 to Tomato
Author(s):
DU Yu-li1; ZHANG Zi-jun2; LI Hai-tao2; ZOU Qing-dao2
1.College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866;
2 Institute of Vegetable,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110866
Keywords:
tomato yellow leaf curl virus disease; Ty-1; molecular marker
PACS:
S436.412.1+1
DOI:
-
Abstract:
Three resistant homozygous lines (Ty-1/Ty-1) and four homozygous susceptible lines (ty-1/ty-1) were amplified by specific primer，both resistant and susceptible lines produced PCR product about 398 bp，after digestion with enzyme Taq I，resistant genotypes and heterozygous resiatant genetypes could produce fragment about 303 bp,98 bp and 398 bp,303 bp and 98 bp respectively.Susceptible genotypes still presented 398 bp fragment.This marker could distinguish resistant and susceptible lines，was a co-dominant marker tightly linked to Ty-1 gene.13 hybrids were detected using this marker，and the results showed that 8 hybrids confer Ty-1 resistant gene and 3 hybrids do not confer Ty-1 resistant gene.The marker also were used to detect 13 inbred lines and all of them do not confer Ty-1 gene.

References:

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Last Update: 2014-09-16