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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年12期

Page:

148-152

Research Field:

Publishing date:

Info

Title:

CloningandSequenceAnalysisofWD₄₀TranscriptionalRegulatorofAcerpalmatum

Author(s):

ANLong-jie ¹; 2; FENGDa-ling²; SHIBao-sheng²

1.NationalEngineeringLaboratoryofForestTreeBreeding，Beijing100000；2.CollegeofLandscapeandTravel,AgriculturalUniversityofHebei，Baoding,Hebei071000

Keywords:

Acerpalmatum; WD₄₀transcriptionalfactor; genecloning; sequenceanalysis

PACS:

S792.35

DOI:

-

Abstract:

WithAcerpalmatumasmaterials，totalRNAwasisolatedfromtheleafofAcerpalmatumandactedastemplateforreversetranscriptionpolymerasechainreaction(RT-PCR).Theresultsshowedthatthefull-lengthcDNAencodingatranscriptionalfactorwasclonedviaRACE-PCRmethodandthecDNAcontainedanORFof1104bpencoding337aminoacids.ThroughBlastsoftwareinNCBI，thenucleotidesequenceandtheaminoacidsequenceoftheleafofAcerpalmatumwereanalyzed.TheresultsshowedthatthenucleotideandaminoacidsequencesofthecDNAinAcerpalmatumwerehighlyhomologouswiththoseoftheWD₄₀transcriptionfactorinotherspecies，andfourconservedWDrepeatmotifwerepresentedintheC-terminalofaminoacidsequences，whichsuggestedthesequencewasWD₄₀ transcriptionfactorgene.

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Last Update: 2014-09-25