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Optimized Study on Growth of Tissue Culture Sprout of Schisandrachinensis(Turcz.)Baill(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年10期
Page:
133-135
Research Field:
Publishing date:

Info

Title:
Optimized Study on Growth of Tissue Culture Sprout of Schisandrachinensis(Turcz.)Baill
Author(s):
ZHANG Zheng-hai; ZHANG Yue; LI Ai-min; LI Yu-huan
Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun,Jilin 132112
Keywords:
Schisandrachinensis(Turcz.)Baill; tissue culture sprout; optimize
PACS:
S 567.9
DOI:
-
Abstract:
Chosen stem section of aseptic seedling of schisandrae new cultivar ‘Red pearl’ as test materials,and MS medium as minimal medium,effect of medium and different concentration NAA and 6-BA on first-generation inoculation and subculture of tissue culture sprout of Schisandrachinensis(Turcz.)Baill were studied by the methods of orthogonal test design f L9（34）.The results showed that the medium MS+0.1 mg/L 6-BA+0.01 mg/L NAA was suitable for the early generation inoculation,the medium MS+0.1 mg/L 6-BA+0.02 mg/L NAA was suitable for subculture and growth.When the ratio of 6-BA/NAA was 5，the rate of callus and the size of callus was moderate and was conducive to tissue culture sprout growth.

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Last Update: 2014-09-05