|Table of Contents|

Study on Callus Induction and Efficient Regeneration System in Different Varieties of Anthurium andraeanum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年10期
Page:
130-132
Research Field:
Publishing date:

Info

Title:
Study on Callus Induction and Efficient Regeneration System in Different Varieties of Anthurium andraeanum
Author(s):
XUE Qi-qin; LI Mei-qin; PEI Hua-li; QIAO Ning; LIU Yong-guang; YANG Tian-hui
Weifang University of Science and Technology，Shouguang,Shandong 262700
Keywords:
Anthurium andraeanum; cllus; induction; plant regeneration
PACS:
S 682.1+4
DOI:
-
Abstract:
The petioles and blade of three Anthurium andraeanum varieties of ‘Fire’,‘Alabama’,‘Pink Champion’ were used as explants，the callus induction and plantlet regeneration of Anthurium andraeanum were studied,and its highly efficient regeneration system were established.The results showed that petioles were suitable explants in callus induction.The optimum medium for callus induction used petioles as explants was 1/2MS medium supplemented with 1.5 mg/mL 6-BA and 0.6 mg/mL 2,4-D,induction rate was 81.7%；The optimum medium for callus induction used blade as explants was 1/2MS medium supplemented with 1.0 mg/ mL 6-BA and 0.8 mg/mL 2,4-D,induction rate was 65.0%；The rates of callus induction and bud differentiation of Anthurium andraeanum ‘Fire’ were highest.And the rates of callus induction and bud differentiation of A.andraeanum “Alabama” were higher than those of A.andraeanum‘Pink champion’.The optimum medium for bud differentiation used petioles and blade as explants were 1/2MS medium supplemented with 1.5 mg/mL 6-BA and 0.6 mg/mL 2,4-D，induction rate were 88.3% and 73.3% respectively；The most suitable rooting medium was 1/2MS medium supplemented with 0.5 mg/mL NAA.Turfy soil and perlite(1∶1) was useful for transplantation，and the survival rate was 95%.

References:

[1]夏春华.世界红掌切花业概况和发展海南红掌切花产业的思路[J].热带农业科学,2001(1):7-9. [2]Pierik R L M,Steegmans H H M,Van Der Meys J A J.Plantlet formation in callus tissues Anthurriurn andraeanum Lind.[J].Scientia Horticulturae,1974(2):193-198.
[3]Pierik R L M.Anthurriurn andraeanum plantlets produced from cultivated in vitro [J].Physiol.Plant，1976,37:80-82.
[4]Pierik R L M,Van Leeuwen P,Pigter G C C M.Regeneration of leaf explants of Anthurriurn andraeanum Lind.in vitro [J].Netrh.J.Aagric.Sci,1979,29:221-226.
[5]肖三元,梁国平,杨焱,等.红掌不同品种产生愈伤组织的差异[J].热带农业科学,2005,28(2):7-9.
[6]兰芹英,仇玉萍,张远辉,等.不同红掌品种的叶片叶柄和茎段愈伤组织的诱导及植株再生[J].西北植物学报,2003,23(6):1006-1009.
[7]兰芹英,李启任,何惠英,等.红掌愈伤组织诱导和芽的分化[J].园艺学报,2003,30(1):107-109.

Memo

Memo:
-
Last Update: 2014-09-05