|Table of Contents|

Study on Establishment of Aseptic System of Malus halliana(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年10期
Page:
126-129
Research Field:
Publishing date:

Info

Title:
Study on Establishment of Aseptic System of Malus halliana
Author(s):
ZHANG Ling-lingGUO Jun-zhanZHANG MinYANG MeiJIANG Xiao-dan
College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100
Keywords:
Malus hallianaexplant disinfectionbud germination
PACS:
S 685.99
DOI:
-
Abstract:
Taking Malus halliana as test material,explant disinfection factors of Malus halliana and the best combination of culture medium and plant growth hormones concentration for bud germination were studied.The results indicated that the explants of best disinfectant effect were those which were young and sampled in April to August every year;the best disinfection method of stem segment with single bud was 75% alcohol 30 s with 0.1% HgCl2 6 min,the best disinfection method of leaf was 75% alcohol 30 s with 0.1% HgCl2 8 min,Because of high infection rate,it was too hard for Petal to establish aseptic system;Orthogonal experimental design showed that the best combination of culture medium and plant growth hormones concentration for bud germination was WPM+6-BA 1.0 mg/L+NAA 0.5 mg/L,and germination rate was 87%.

References:

[1]刘志强,汤庚国.海棠在园林中的应用研究[J].苏州科技学院学报(工程技术版),2004,17(3):75-80.
[2]许晓岗,童丽丽,赵九洲.垂丝海棠插穗的内源激素水平及其与扦插生根的关系[J].江西林业科技,2007(1):20-24.
[3]李晓东,许元峰,李文娟,等.垂丝海棠快速繁育[J].林业实用技术,2002(11):23-24.
[4]王嘉祥.垂丝海棠的栽培[J].特种经济动植物,2005(4):34.
[5]陈相国,李晓东,许元峰,等.垂丝海棠嫁接繁育技术[J].山东林业科技,2003(2):29-30.
[6]张庆田,夏阳,孙仲序,等.垂丝海棠组培再生体系建立的研究[J].生物技术,2007,17(3):73-75.
[7]马兰珍,韦力秀,薛鹰,等.杂交松组织培养中外植体的灭菌方法[J].广西科学院报,2005,21(1):37-39.
[8]徐振彪,傅作中.植物组织培养中的褐变现象[J].植物生理学通讯,1997,5(1):55-56.
[9]李浚明.植物组织培养教程[M].北京:中国农业大学出版社,1998:215-312.
[10]袁红霞,王金虎,陈德燕.何首乌块根愈伤组织培养[J].苏州大学学报,2005,21(3):85-88.
[11]包振华,郭军战,周玮,等.枸杞组织培养再生体系优化[J].西北林学院学报,2010,25(5):73-76.
[12]马冬菁,叶景峰,陈罡,等.贴梗海棠组织培养技术研究初报[J].辽宁林业科技,2007(4):51-52.
[13]戴全胜,王雅群,车晓航,等.颐和园古西府海棠的组织培养与快速繁殖[J].林业科技开发,2010,24(2):73-76. [14]梁美霞,戴洪义.苹果组织培养苗离体叶片诱导不定芽分化[J].湖南农业大学学报(自然科学版),2009,35(5):470-473.

Memo

Memo:
-
Last Update: 2014-09-05