Taking trees leaves of Platanus×acerifolia and Populus canadensis as raw materials，through the different treatment for new type of bio-medium;taking petunias,string,marigold seeds as test materials，hole tray seeding；the seeds sprout，seedling growth power，leaf area and the growth of root vigor physiological were studied.The results showed that the matrix of Populus canadensis was beneficial than Platanus×acerifolia to a variety of flowers growth and development，especially the treatment with leaves∶drying chicken∶fermentation bacteria∶water = 3 000∶180∶10∶2 000 received bio-medium，the effect was better.