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Effect of Pretreatment with PP333on Culture Directly into Seedling to Lonicera japonica var.chinensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年09期
Page:
184-185
Research Field:
Publishing date:

Info

Title:
Effect of Pretreatment with PP333on Culture Directly into Seedling to Lonicera japonica var.chinensis
Author(s):
WANG-Wen-jingLI Wei-qiangWANG Peng
Zhengzhou College of Animal Husbandry Engineering,Zhengzhou ,Henan 450011
Keywords:
Lonicera japonica var.chinensisPP333pre treatmentexplantculture medium
PACS:
S 685.99
DOI:
-
Abstract:
With a concentration of 0, 200, 500 mg/kg respectively of PP333on potted plants of Lonicera japonica var.chinensis one month in advance were treated to the root, and then the bud and stem segment with axillary bud after PP333 treatment were connected to culture to the most suitable culture medium after eradicating bacterium. The effect of pretreatment with PP333on culture directly into seedling to Lonicera japonica var.chinensis were studied. The results showed that all the explants were germinated, injury organization gradually appeared after 10~15 d; the leaves, and stems were normal pumping after 35~45 d; the branches with PP333 pretreatment were sprouting more than CK, the internodes were short, the leaves were dark green, the leaf area was small than CK after 60~70 d. The growth of the tissue culture seedlings of all the treatments were different significantly, the stems and leaves of the culture seedlings with 200 mg/kg treatment were stout,and 500 mg/kg followed by, CK was the worst.

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Last Update: 2014-09-03